High performance liquid chromatography is central to pharmaceutical, food, water and petrochemical testing throughout African laboratories, yet method transfer between instruments and operators often introduces variability. This course develops sound HPLC practice, covering mobile phase preparation, column chemistry, detector selection and system suitability testing, so analysts generate consistent, defensible results across shifts and sites.
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Explain the principles of liquid chromatography separation and common detector technologies.
Prepare mobile phases and select columns appropriate to the analyte and matrix.
Perform system suitability testing before releasing quantitative HPLC results.
Operate HPLC systems for isocratic and gradient methods across sample types.
Troubleshoot pressure faults, peak shape problems and retention time drift.
Validate transferred methods to confirm equivalent performance between instruments and operators.
Course outline
01Liquid Chromatography Separation Principles
02Mobile Phase Preparation and Degassing
03Column Chemistry and Selection
04Detector Types and Selection Criteria
05Gradient and Isocratic Method Operation
06System Suitability Testing
07Troubleshooting Pressure and Peak Faults
08Method Transfer Between Instruments
Who should attend
This course is intended for HPLC analysts, method development chemists, quality control staff and instrument technicians working in pharmaceutical, food, water or petrochemical laboratories.
Fees are per delegate. South African venues are priced in rand; other locations and online in US dollars. Fees exclude 15% VAT where applicable. Group bookings of three or more delegates from the same organisation qualify for a reduced rate; contact us for a quotation.
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